Mouse Lines

The Blakely Lab has created the following mouse lines. References detailing the generation of each of the lines are included below.

For inquiries, please contact Dr. Blakely directly.

SERT Ala56
- Gly56Ala variant is associated with autism
- Veenstra-VanderWeele J, Jessen TN, Thompson BJ, Carter M, Prasad HC, Steiner JA, Sutcliffe JS, Blakely RD. Modeling rare gene variation to gain insight into the oldest biomarker in autism: construction of the serotonin transporter Gly56Ala knock-in mouse. J Neurodev Disord. 2009 June; 1(2):158-71, [PMID: 19960097, PMCID: PMC2786076]

SERT I172M
- I172M variant disrupts high-affinity binding of antidepressants and cocaine
- Thompson BJ, Jessen T, Henry LK, Field JR, Gamble KL, Gresch PJ, Carneiro AM, Horton RE, Chisnell PJ, Belova Y, McMahon DG, Daws LC, Blakely RD. Transgenic elimination of high-affinity antidepressant and cocaine sensitivity in the presynaptic serotonin transporter. Proc Natl Acad Sci USA. 2011 Mar1;108(9):3785-90, [PMID: 21282638; PMCID:PMC3048100]

DAT Val559
- DAT A559V variant is associated with bipolar disorder and ADHD
- Mergy MA, Gowrishankar R, Davis GL, Jessen TN, Wright J, Stanwood GD, Hahn MK, Blakely RD. Genetic targeting of the amphetamine and methylphenidate-sensitive dopamine transporter: On the path to an animal model of attention-deficit hyperactivity disorder. Neurochem Int. 2013 Dec 8, ePub ahead of print, [PMID: 24332984]

Floxed IL-1R
- Conditional elimination of interleukin-1 receptor
-Available from Jackson Labs, 028398
- Robson MJ, Zhu CB, Quinlan MA, Botschner DA, Baganz NL, Lindler KM, Thome JG, Hewlett WA, Blakely RD. Generation and Characterization of Mice Expressing a Conditional Allele of the Interleukin-1 Receptor Type 1, PLoS One. 2016 Mar 1;11(3):e0150068. doi: 10.1371/journal.pone.0150068. PMC4773179

CHT KO
- Knockout of the hemicholinium-3 sensitive choline transporter
- Homozygous deletion of CHT is lethal
-Available from Jackson Labs, 028692
- Bazalakova MH, Wright J, Schneble EJ, McDonald MP, Heilman CJ, Levey AI, Blakely RD. Deficits in acetylcholine homeostasis, receptors and behaviors in choline transporter heterozygous mice. Genes Brain Behav. 2007 Jul;6(5):411-24 [PMID: 17010154]

BAC-CHT
- Multiple copies of choline transporter gene
- Available from Jackson Labs, 028695
- Holmstrand EC, Lund D, Cherian AK, Wright J, Martin RF, Ennis EA, Stanwood GD, Sarter M, Blakely RD. Transgenic over expression of the presynaptic choline transporter elevates acetylcholine levels and augments motor endurance.Neurochem Int. 2013 Nov 22, ePub ahead of print, [PMID: 24274995]

CHT Motorneuron Overexpressing Mice
- CHT +/+ and CHT -/- expressed under control of the motor neuron promoter Hb9
- Lund D, Ruggiero AM, Ferguson SM, Wright J, English BA, Reisz PA, Whitaker SM, Peltier AC, Blakely RD. Motor neuron-specific over expression of the presynaptic choline transporter: impact on motor endurance and evoked muscle activity. Neuroscience. 2010 Dec 29;171(4):1041-53, [PMID: 20888396; PMCID: PMC2992794]

ProT KO
- Proline Transporter knock-out mouse
- More information available soon