Needle Sizes and Recommended Injection Volumes

Species Intravenous Intraperitoneal Intramuscularly Subcutaneous
Mouse Lateral tail vein, 0.2 ml, < 25 gauge 2-3 ml, < 21 gauge Quadriceps/posterior thigh, 0.05 ml,< 23 gauge Scruff, 2-3 ml, < 20 gauge
Rat Lateral tail vein, 0.5 ml, < 23 gauge 5-1 0 ml, < 21 gauge Quadriceps/ posterior thigh, 0.3 ml, < 21 gauge Scruff, back, 5-1 0 ml, <20 gauge
Hamster Femoral or jugular vein (cut down), 0.3 ml, < 25 gauge 3-4 ml , < 21 gauge posterior Quadriceps/ posterior thigh, 0.1 ml, < 21 gauge Scruff, 3-4 ml,< 20 gauge
Guinea pig Ear vein, saphenous vein, 0.5 ml, < 23 gauge 10-15 ml, < 21 gauge Quadriceps/ posterior thigh, 0.3 ml, < 21 gauge Scruff, back 5-10 ml, < 20 gauge
Rabbit Marginal ear vein, 1-5 ml (slowly), < 21 gauge 50-100 ml, < 20 gauge Quadriceps/ posterior thigh, lumbar muscles, 0.5-1 ml, < 20 gauge Scruff, flank 30-50 ml, < 20 gauge
Cat Cephalic vein, 2-5 ml (slowly), < 21 gauge 50-1 00 ml, < 20 gauge Quadriceps/ posterior thigh, l ml, < 20 gauge Scruff, back 50-100 ml, < 20 gauge
Dog Cephalic vein, 10-15 ml (slowly), < 20 gauge 200-500 ml, < 20 gauge Quadriceps/ posterior thigh, 2-5 ml, < 20 gauge Scruff, back, 100-200 ml, < 20 gauge

'Modified from Flecknell, P.A. Laboratory Animal Anesthesia, an introduction for Research workers and Technicians, Academic, Press, Orlando, Florida,1987.

From the Training Manual Series. Vol. 2, Laboratory Animal Technician. 1990 ed. AALAS.

1998 AALAS Reference Directory 91

August 2006






Privacy Policy | University Regulations and Policies | Emergency Information | Get Help | Contact Us
An Equal Opportunity/Equal Access Institution

© Copyright 2012. Florida Atlantic University.
 Last Modified 11/16/12